Antibodies - Annexin V - Cytokines - ELISA

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An antibody (Ab) or immunoglobulin (Ig) is a glycoprotein produced mainly by plasma cells in adaptive immune system. Antibody plays an important role in neutralizing the pathogens such as bacteria, fungi and viruses and allergic substances by using the specificity region for each epitope. Antibodies have been classified into each isotype base on structure IgA, IgG, IgD, IgE, and IgM. 


The secreted proteins in immune system produced by various types of cells and have effects on immune cells. Binding of cytokines and its receptors on target cells lead to signal transduction and immune response. Cytokines have been classified into many types base on the functions of each cytokines. 


Enzyme–linked immunosorbent assay (ELISA), a technique used for antigen detection in soluble sample. A principle of this technique is the specificity of antibody to antigen combine with the enzyme-colorimetric technique to quantified the amount of target antigen in liquid sample such as plasma and condition media. 

Annexin V

Apoptotic cells change the structure of their membrane, which leads to the exposure of phosphatidylserine (PS) on the membrane surface Annexin V and its conjugates can be used for the detection of apoptosis because they interact strongly and specifically with exposed phosphatidylserine. Labeled annexin V with fluorescent dye can be used for apoptotic cells detection by immunofluorescence and flow cytometry technique

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